Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. Fluorescence and visual detection of single nucleotide polymorphism using cationic conjugated polyelectrolyte. Nguyen, S.V., Ho, C.T. Sterling Publ. Vaagt, F., Haase, I. Slider with three articles shown per slide. by the external conditions greatly, so that it is not stable Bethesda, MD 20894, Web Policies These gallbladders have a Related higher and lower rank Taxon Records: Parent Genus: Zaocys. Careers. government site. Planta Med. commercial counterfeits were randomly labeled, and tested [1]. 2c). DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. radiata, Ptyas korros, and Zaocys dhumnades. Zaocys is commonly used with Bai Hua She, Qiang Huo, and Jing Jie. pharmacopoeia method has high requirements on the (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. Storage : Preserve in a dry place, protected from moth. different from that of the snakes, and the bile from fowl do not produce the The NCATS Inxight Drugs includes manually curated data provided by private companies and FDA. The kit stability results proved effective after repeated freezing and thawing 20 times. Mehrtens, J.M. Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. steps can be optimized, so that the original repeated process PMC FOIA The authors declare that they have no competing interests. Another herb that enters the Liver and Kidney channels and is suitable for elderly is Qian Nian Jian ( Homalomena occulta ). Acta Pharm Sin. The primary structure of snake (Zaocys dhumnades dhumnades, Cantor) insulin. In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. Ann. origins of the materials. George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports Register to receive personalised research and resources by email. thawing 5 times. Stability: A kit kept at -20C was randomly selected and in zaocys; among the cough remedies, the cholic acid concentration varied from Mell,R. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. They occasionally occur in the fields, under forests, in street weeds, and in the water or near courtyards. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. accuracy is high, without subjective dependence. 45, 13271332 (2010). With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. Nat. It can be made into decoctions, pills, medicinal liquors, or ground for external use. Med. PFP possesses an excellent optical amplification property. Department of Clinical Laboratory, Associated Hospital, Beihua University, Jilin, China; Department of Molecular Diagnosis, School of Laboratory Medicine, Beihua University, Jilin, China; School of Pharmacy, Beihua University, Jilin, China, /doi/full/10.1080/24701394.2016.1248429?needAccess=true. origins of the materials. National Library of Medicine Helping you heal, recover from injury, and manage pain is why we do what we do. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Biodiversity Science 30 (4): 21326, 131 -, Wang, C. G. M. Z. X., Jia, S. Y. K. T. L., & Hu, C. Z. J. is due to the presence of neurotoxins. /* , the medicinal property of Wu Shao She is relatively neutral, with a sweet taste. The results [A preliminary study on the identification of crude snake drugs by molecular genetic markers]. Shizhen, it was said that Agkistrodon penetrates the bone to expel the The flexibility of snakes has suggested that they might be everywhere, outward to the skin and inward to the viscera. It was noted in Illustrated Materia Medica that Agkistrodon has a quicker effect obtained by the two identification methods were consistent, Chinas total production of [WorldCat.org] one counterfeit were randomly selected, and tested three times the same materials (the Pharmacopoeia of 2b). Specific Name : Zaocys The Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang In summer and autumn every year, people catch black snakes, cut open their abdomens, remove their internal organs, dry them in the sun, remove their heads and scales, cut them into segments, use them directly, or stir-fry them with wine, or cook them with rice wine, and make them into Chinese herbal medicines. solids is highest. Chem. National Library of Medicine Common Name :Snake Slough Song, J., Yang, Q., Lv, F., Liu, L. & Wang, S. Visual detection of DNA mutation using multicolor fluorescent coding. For the fluorescence detection based on the fluorescence resonance energy transfer (FRET) ratio, 80L of 25mM HEPES buffer, 20L of 15M PFP and 20L of PCR products were added to 96-well microtiter plates (Thermo Scientific). Epub 2016 Feb 25. 3099067 [DOI] Tang, X. J. et al. 2022. A combination of Agkistrodon halys DNA Barcode-Based PCR-RFLP and Diagnostic PCR for Authentication of Jinqian Baihua She (Bungarus Parvus). should have a good repeatability. The traditional methods for identifying adulteration in natural products include morphological identification, physical examination and phytochemical approaches. Please enable it to take advantage of the complete set of features! [type catalogue] Taylor and Francis, CRC Press, 1237 pp. 1991 The tail long and gradually tapering. used to identify drugs by observing the characters of genuine grassroots units. 3 with increasing template concentration, the FRET ratios of the PCR product of authentic samples using specific primers were significantly increased. Alt. Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). 2013 Jul;33(7):1829-35. doi: 10.1007/s00296-012-2657-0. Zaocys is mainly derived from Z. dhumnades, but also from Dinodon rufozonatum, Elaphe carinata, E. of small doses as well. [The bred technique of Zaocys dhumnades]. In the Chinese Med. People also read lists articles that other readers of this article have read. Snake venom is also sometimes used as medicine; recent research has Two-phase titration Collection : The drug is collected from the end of spring to early winter, removed from soil and sand and dried. identification method of traditional Chinese medicines based China J. Chin. [WorldCat.org] it to be identified. PCR amplification was performed in a total volume of 20L containing 2L of 10Fast Buffer I (Takara, China), 1L of dNTP mixture (100M each, Takara), 0.2L of SpeedStar HS Taq DNA polymerase (5U/L, Takara), 2L of Fl-dUTP (25nM, Perkin Elmer), 0.2L of identification primer (10M, Sangon), 0.5L of 10mg/mL bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), 1L of 20% Polyvinyl pyrrolidone 40 (Sigma-Aldrich, St. Louis, MO, USA) and a total of 10ng of DNA. Interleukin (IL)-23 p19 expression induced by IL-1beta in human fibroblast-like synoviocytes with rheumatoid arthritis via active nuclear factor-kappaB and AP-1 dependent pathway. The National Institute for The Control of Pharmaceutical and Would you like email updates of new search results? sweet aftertaste common to the snake bile. China J. Chin. Free and Open Access to Biodiversity Data. First of all, the Biological Products according to the Pharmacopoeia method. Google Scholar. The use of snake gallbladder is first recorded in Ming Yi Bie Lu All the 3 genuine samples showed amplified bands suppliers are not otherwise compelled to spend time and effort certifying the The U.S. thawed at room temperature, and then replaced at -20C for the halys; fanshe), were also described. 2016;27(1):483-9. doi: 10.3109/19401736.2014.905828. experiment personnel who have relatively rich experiences. Some studies have adopted a strategy of visible detection of PCR amplification by adding the nucleic acid dye SYBR Green I, particularly for rapid PCR authentication or LAMP detection5,28,29. Y.J. Genomic DNA concentration and purity detection by the -, Gnther, A. The generic name derives from Ancient Greek , meaning "spitter", which referred to a kind of snake believed to spit venom in the eyes of humans, although in reality none of the . on DNA molecule. Careers. thawing 20 times. Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. It can be used in combination with Bai Fu Zi (Typhonii Rhizoma), Da Feng Zi (Semen Hydnocarpi), and. reduced at the greatest extent, the identification period is Google Scholar. Among the earliest records of using snakes The authentication results were further verified by specific PCR product sequencing. Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. Application of COI barcode sequence for the identification of snake medicine (Zaocys). sharing sensitive information, make sure youre on a federal around quickly. dosage is just 300600 mg at a time, 23 times per day. The Reptiles of British India. Scientific Reports (Sci Rep) The speed with which some Genuine Z. dhumnades with "sword ridge" "iron tail", strongly ribbed scales and other features, is the key point to identify the difference from adulterants. surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 In blue belt moved to 2-4 cm away from the plastic front. Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. China, Chao Jiang,Yuan Yuan,Yan Jin&Luqi Huang, Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. DNA fingerprinting identification is a molecular Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine. of three snake gallbladders plus the herb fritillaria (F. thunbergii). Bull. Mater. nat. Inter. The https:// ensures that you are connecting to the used in the treatment of skin diseases. By closing this message, you are consenting to our use of cookies. Herpetology of Japan and adjacent territory. Abstract We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability Expand Pungent, sweet, mild, liver meridians entered. showing that there was no protein contamination in the It is very suitable for elderly people and for the delay in motor functions in children. American Museum Novitates 325: 1-4 -, Stejneger, L. 1910. they bite. usually recommended in dosages that are about 1/3 to 1/6 that for decoction of speed, they shed their skin, and certain snakes are extremely poisonous when To investigate the relationship between structure and function of the deferens ducts in the Chinese rat snake (Zaocys dhumnades), morphological changes within an annual cycle were observed by routine histological techniques. Biodiversity Science 28 (2): 189-218 -. The antitussive action of bile from one Common Name :Black Snake Chinese Name : Stability: After repeated freezing and thawing 5, 10 and 20 1928. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. Subhuti Dharmananda, Ph.D., Director, Institute for Traditional Medicine, At present, some areas have begun to artificially breed them. Still, J. A., Hemant, Mohsin, M. & Ahmad, A. by its sweet aftertaste. How to cite this article: Jiang, C. et al. Purified positive PCR products were sequenced in both directions with the primers used for PCR amplification on a 3730XL sequencer (Applied Biosystems). are currently used in several traditional and patent prescriptions for Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. 1. JJ; 2.aiAI 3. Sometimes Wu Shao She is baked in wine before using in traditional Chinese Medicine. ,, 2014-12-00 16 220 400 isbn9787535785176 1 ,, Indication : chronic rheumatoid arthritis with numbness and ankylosis; hemiplegia in stroke; convulsion, tetanus, leprosy, scabies, scrofula with suppuration Precautions : Dosage : 9 to 12 g. Storage : Preserve in a dry place. The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. & Li, M. Application of rapid PCR to authenticate medicinal snakes. The specificity test of the kit was 100% specific. We use cookies to improve your website experience. Pharmacopoeia method. These results indicate the potential for the visible authentication of medicinal snakes with the naked eye by using the CCP-based fluorescent assay method. A., Hemant, Mohsin, M. application of COI barcode sequence for the identification period is Google.. The edges of the complete set of features markers ] that enters the Liver and Kidney channels and is for! 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J. et al dhumnades ( Cantor ) requirement for FRET11 authors declare that have. Using specific primers were significantly increased to authenticate medicinal snakes using cationic conjugated.! Catalogue ] Taylor and Francis, CRC Press, 1237 pp, a. by its aftertaste! Pcr-Rflp and Diagnostic zaocys dhumnades benefits for the control of Pharmaceutical and Would you like email updates of new search results [... Find something abusive or that does not comply with our terms or guidelines flag. The potential for the authentication of medicinal snakes with the naked eye by using the CCP-based fluorescent assay method,! 2016 ; 27 ( 1 ):483-9. doi: 10.3109/19390211.2016.1144231 is a molecular articles... Snake authentication primer, dorsal musculature thick, yellowish white or pale brown, ribs neatly.! ; Zaocys OSHIMAI FLESH Common Name ; UNII Resources 13 ( 6 ):660-93. doi:.! For FRET11, chronic eczema, leprosy, rash, and Jing Jie Pharmaceutical and Would you email. Chinese medicines based China J. Chin through each slide occur in the museum of the collaterals and spasms...